Composite

Part:BBa_K3583011

Designed by: Sebastian Kusnik   Group: iGEM20_Duesseldorf   (2020-10-22)


Escherichia coli PPK mutant E173K for expression in Physcomitrella patens

This construct was designed for expression in the moss Physcomitrella patens. It starts with the highly effective I2-10 promoter, followed by a RBS, the enzyme and the nos terminator. For more information on the single parts, check the information given on the pages of each biobrick.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 75
    Illegal XbaI site found at 146
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 121
    Illegal NheI site found at 168
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 897
    Illegal BglII site found at 2647
    Illegal BamHI site found at 353
    Illegal XhoI site found at 348
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 75
    Illegal XbaI site found at 146
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 75
    Illegal XbaI site found at 146
    Illegal AgeI site found at 2251
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None